RESUME


Kathy Bailey
Phone:  (415)514-0241
e-mail: krb@phy.ucsf.edu
June, 1982 BS, Biochemistry
Department of Biochemistry
University of California
Los Angeles, California 90024

May, 1995Analytical & Light Microscopy
Woods Hole

February, 1996Flow Cytometry
Laboratory Medicine, UCSF


SUMMARY OF RESEARCH EXPERIENCE
I have demonstrated an ability to conduct experiments independently and present the results of these experiments in several areas of biomedical research:

Genetics: I have studied the transforming ability of and performed RFLP analysis on various oncogenes; have studied the transcriptional regulation of proteins expressed during adrenal medula development; conducted mutational analysis of the ara operon; identified specific transposon Tn7 target insertions; and have cloned cDNAs and genomic DNAs of cGMP-gated channels, lectins, and oncogenes from lambda and cos libraries and/or pcr.

Biochemistry: I have broad experience in purifications and analysis of proteins and protein modifications (glycosylation and phosphorylation), i.e., collagens, lectins, oncoproteins, cytokeratins, monoclonal and polyclonal antibodies to these proteins.

Cell Biology: I have prepared and characterized numerous antibodies and used them in immuno-histochemical studies and assays; have developed and performed patient study assays for Scavenger Receptors, TGF, HTLV I, SK, NK, cytokeratins, oncoproteins; and have cultured and minipulated primary retinal pigment epithelial cells, rat brain cells, bone marrow cells and numerous eucaryotic cell lines as required for studies.

SUMMARY OF ADMINISTRATIVE EXPERIENCE
I have been a project leader responsible for development of cancer diagnostic reagents (anti-oncogene, anti-HTLV and cytokeratin antibodies and immuno- histochemistry reagents); and I validated claims of reagents and coordinated collaborations among independent research labs and the marketing group at Triton Biosciences.

I have coordinated all phases of laboratory build-outs for three laboratories (molecular biology, biochemistry and diagnostics); this included ordering all laboratory equipment, hiring and training research assistants/associates; training of post-doctoral fellows and physicians; planning and implementing budgets; and organizing a Mac computer network and developing databases for them.

COMPUTER SKILLS: Certification, Computer Networking, San Francisco City College: UNIX, DOS, LANs ( including networking of Macs and PCs (Novell); most computer programs (Excel, PhotoShop, Prism statistical analysis, image analysis programs for gel, photo-microscopy and FACS analysis); Perl programming and Web authoring.

INSTRUMENTATION UTILIZED: FLOW CYTOMETER; ANALYTICAL AND LIGHT MICROSCOPY; AUTOMATED SEQUENCER, ALF; OLIGONUCLEOTIDE SYNTHESIZER: Protocols and chemistries; COLUMN CHROMATOGRAPHY--; ion exchange, affinity and HPLC); Electrophoresis (2-D gels, westerns, etc.)

(1992-2000)I was the Electronic Seminars Publication and Web site manager at UCSF:


EMPLOYMENT HISTORY
4/'96-present Metabolic Research & Ophthalmology
UCSF		 Dan Schwartz, MD
Keith Duncan, PhD
415/514-0241

I have identified scavenger receptors and investigated gene regulation by lipid & modified lipid, i.e. oxidized-lipid uptake in human retinal epithelial cells; and have tested drugs which modulate the uptake. Lipid and modified lipid assays were used to devolop assays that simulate photoreceptor outer segment uptake. I identified isoforms of thyroid hormone and RXR receptors and other genes in the trabecular meshwork which may be involved in modulating ocular pressure observed in glaucoma and retinal degeneration by molecular, immunohistochemical and assay analysis. I have participated in studies on gene regulation of renin, i.e., renin promoter studies for specific chorion cell types and mutagenesis.

Jul '94 - Jul '96 CORE MANAGER
Dept of Neurology, Gallo Center
SFGH, Building 1, Room 101
San Francisco, CA 94110
 Mike Miles,MD
415/648-7111

The center studies the effect of alcohol on physiology, cell biology and genes. I managed the core facility which performed automated sequencing projects and oligonucleotide synthesis; and engineers and prepares constructs for expression of fusion proteins.

1992-6/'94 RESEARCH ASSOCIATE
Dept Physiology & Biochemistry/Biophysics
UCSF		 Juan Korenbrot,PhD
(415)476-4601

The lab studies signaling in the retina and retinal cell lineages. I cloned cGMP gated ion channels in rods and cones of striped bass and expressed the wild type channels for physiological studies.

1990-'91RESEARCH ASSOCIATE
Neurosurgery/BTRC
UCSF		 Mark Israel,MD

The lab studied gene regulation in neural development, astrocyte function and genetics. I investigated gene regulatory sequences in the adrenal medulla and managed all aspects of the laboratory. Experimental proposals, journal clubs and results were prepared and presented regularly.

1986-1990RESEARCH ASSOCIATE
Dept of Psychiatry
UCSF		 Samuel Barondes,MD
Dir Mol Genetics
UCSF
(415)476-7066

The lab focused on the characterization and function of lectins. I investigated the role of endogenous lectins in vertebrate tissues and performed the biochemistry and molecular biology required for the study. I managed the administrative and operational aspects of the lab.

1984-'86PROJECT LEADER
Triton Biosciences, Inc
Alameda, CA		 Boyce Burge,PhD
Manager,Diagnostics
(415)361-8662

Our group developed cancer diagnostics and clinical assays. I was project leader for cancer diagnostics reagents. I collaborated with research and business teams to transfer and develop research reagents. Procedures for patents, clinical trials, quality control and protocol development were established. Reagents/assays included cytokeratin antibodies/assays, HTLV I antibodies/assays, anti-oncogene and anti-TGF antibodies/assays.

1982-'85RESEARCH ASSOCIATE
Hematology/Oncology, UCLA		 Martin Cline,MD
(310)825-7865
Dennis Slamon,MD,PhD
(310)825-5193
The lab was involved in developing cancer diagnostics. I investigated potential cancer diagnostics,i.e., oncogene protein assays (using RIPA and IEF gels), performed RFLP's and TGF assays for Triton Biosciences, generated and characterized anti-oncogene antibodies and generated oncogene constructs for transfecting cells.

1980-'82RESEARCH ASSOCIATE
Dept Immunology & Microbiology
UCLA		 John Fahey,MD
(510)825-5661

I assisted in the development of immunological assays for patient studies, performed immunofluorescence typing of lymphocytes, monitoring immunological response by ADCC/SK, PHA, specific radioimmunoassay and B cell plaque assay in a clinical laboratory setting. Isolated cells from patient sample and monitored blood cell types and counts manually and with cell sorting techniques.


PUBLICATIONS:
Ishida, BY, Keith G Duncan, Kathy R Bailey, John P Kane, and Daniel M Schwartz, "High Density Lipoprotein - mediated lipid efflux from retinal pigmented epithelial; Cells in Culture"; BJOPHTHALMOL/2005/08576.
Brian Y. Ishida, Kathy R. Bailey, Keith G. Duncan, Robert J. Chalkley, A.L. Burlingame, John Kane, Daniel M. Schwartz, "Regulated Expression of Apolipoprotein E by Human Retinal Pigment Epithelial Cells", Journal of Lipid Research, 16 October.
Bailey, K.R., B.Y. Ishida, K.G. Duncan, J.P. Kane, D.M. Schwartz. 2003, Apolipoprotein E Containing High Density Lipoproteins can Function as Preferential Acceptors for Retinal Pigment Epithelium Cell Digested Photo Receptor Outer Segment Lipids, Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting. Abstract #477.
Stewart, J.M., K.R. Bailey, K.G. Duncan, J.P. Kane, D.M. Schwartz. 2003. Expression of Reverse Cholesterol Transport Proteins SR-B1, SR-BII and ABCA1 in the Human Retinal Pigment Epithelium, Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting. Abstract #1711.
Keith G. Duncan, Kathy Bailey, John P. Kane and Daniel M. Schwartz, Biochemical and Biophysical Research Communications (March 2001)
Keith G. Duncan, Michele D. Jumper, Ralff C.J. Ribeiro, Kathy R. Bailey, Paul M. Yen, Akira Sugawara, Ashmi Patel, Robert Stern, William W. Chin John D.Baxter and Daniel M. Schwartz, "Human Trabecular Meshwork Cells: A Thyroid Hormone Target Tissue." Graefe's Archive of Clinical and Experimental Ophthalmology 1999:237:231-240.
K.G. Duncan, K.R. Bailey, J.D. Baxter and D.M. Schwartz. "The Human Retinal Pigment Epithelium: A Target Tissue for Thyroid Hormones." Ophthalmic Research 1999:31:399-406.
PATENT(S):
ANALYTICAL & LIGHT MICROSCOPY COURSE, WOODS HOLE (1995) This is an intensive summer course to learn the proper use of analytical microscopes, i.e, confocal, light, etc.; staining optimization protocols and technologies; image analysis; etc.
AUTOMATED SEQUENCER, ALF
OLIGONUCLEOTIDE SYNTHESIZER: Protocols and chemistries
Computer (IBM & Mac), including networking of Macs and PCs (Novell)
Phlebotomy license
Cell sorting of fluorescenated lymphocutes
Electrophoresis (2-D gels, westerns, etc.)
Molecular biology (Northerns, Southerns, Westerns; Sequencing; database sequence analysis; PCR; recombinant DNA procedures; retroviral, bacteriophage, baculoviral and bacterial vectors; mutagenesis); RFLP's in genetic analysis, tranfections and bacteriophage transductions; gel shifts.
Immunochemistry (Eliza and RIA assay; TGF and EGF receptor assay, cytokeratin, HTLV)
Tissue culture (neural cell lines; fractionation/characterization of cells from patient sera; ADCC/SK/tritium uptake assays; cell protein labelling; hybridoma technology; primary cell lines, chemical mutagenesis on C3H10T-1/2; oncogene transformation; bone marrow.
Protein biochemistry (phosphopeptide and phosphoaminoacid analysis; chromatography;ion exchange, affinity and HPLC).